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人白介素2酶免試劑盒,(IL-2)ELISA檢測(cè)試劑盒 ,英文名:IL-2 ELISA Kit ,英文縮寫:IL-2 ,常見(jiàn)試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人白介素2ELISA檢測(cè)試劑盒,IL-2 檢測(cè)試劑盒, IL-2 檢測(cè)試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。人白介素2酶免試劑盒,(IL-2)ELISA檢測(cè)試劑盒 操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測(cè)定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測(cè)中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競(jìng)爭(zhēng)法及定量檢測(cè)影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過(guò)程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無(wú)關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過(guò)程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測(cè)讀儀(酶標(biāo)儀),比色法判讀可選擇單波長(zhǎng)或雙波長(zhǎng),根據(jù)反應(yīng)液顏色的不同選用不同波長(zhǎng)的濾光片,以空白孔校零測(cè)定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
雞卵黃免疫球蛋白(IgY)ELISA試劑盒Hen egg Immunoglobulin of Yolk,IgY ELISA KitY0300196T/48T
雞蛋卵黃高磷蛋白磷酸肽(PPP)ELISA試劑盒Hen egg phosvitin phosphopeptides,PPP ELISA KitY0300296T/48T
雞脂多糖/內(nèi)毒素(LPS)ELISA試劑盒Chicken Lipopolysaccharides,LPS ELISA KitY0300396T/48T
雞β干擾素(IFN-β/IFNB)ELISA試劑盒 Chicken Interferon β,IFN-β/IFNB ELISA Kit Y0300496T/48T
雞免疫球蛋白E(IgE)ELISA試劑盒Chicken Immunoglobulin E,IgE ELISA KitY0300596T/48T
雞免疫球蛋白A(IgA)ELISA試劑盒 Chicken Immunoglobulin A,IgA ELISA KitY0300696T/48T
雞腫瘤壞死因子α(TNF-α)ELISA試劑盒Chicken Tumor necrosis factor α,TNF-α ELISA Kit Y0300796T/48T
雞白介素1β(IL-1β)ELISA試劑盒Chicken Interleukin 1β,IL-1β ELISA KitY0300896T/48T
雞主要組織相容性復(fù)合體(MHC/B)ELISA試劑盒Chicken major histocompatibility complex,MHC/B ELISA Kit Y0300996T/48T
雞熱休克蛋白70(HSP-70)ELISA試劑盒 Chicken Heat Shock Protein 70,Hsp-70 ELISA Kit Y0301096T/48T
雞白痢抗體檢測(cè)(PD)ELISA試劑盒 Chiken Pullorum Diseaes,PD ELISA KitY0301196T/48T
雞分泌型免疫球蛋白A(SIgA)ELISA試劑盒Chicken secretory immunoglobulin A,SIgA ELISA KitY0301296T/48T
雞α干擾素(IFN-α)ELISA試劑盒 Chicken Interferon α,IFN-α ELISA KitY0301396T/48T
雞白介素18(IL-18)ELISA試劑盒 Chicken Interleukin 18,IL-18 ELISA KitY0301496T/48T
雞白介素1(IL-1)ELISA試劑盒 Chicken Interleukin 1,IL-1 ELISA KitY0301596T/48T
雞病毒性腸炎病毒(DEV)ELISA試劑盒 Chicken virus enteritis,DEV ELISA KitY0301696T/48T
雞肌紅蛋白(MYO/MB) ELISA試劑盒Chicken Myoglobin,MYO/MB ELISA Kit Y0301796T/48T
雞白介素9(IL-9)ELISA試劑盒Chicken Interleukin 9,IL-9 ELISA Kit Y0301896T/48T
雞白介素3(IL-3)ELISA試劑盒Chicken Interleukin 3,IL-3 ELISA Kit Y0301996T/48T
雞白血病抑制因子(LIF)ELISA試劑盒 Chicken Leukemia inhibitory factor,LIF ELISA Kit Y0302096T/48T
雞轉(zhuǎn)化生長(zhǎng)因子β1(TGF-β1)ELISA試劑盒 Chicken Transforming Growth factor β1,TGF-β1 ELISA Kit Y0302196T/48T
雞C反應(yīng)蛋白(CRP)ELISA試劑盒 Chicken C-Reactive Protein,CRP ELISA Kit Y0302296T/48T
雞血管內(nèi)皮細(xì)胞生長(zhǎng)因子(VEGF)ELISA試劑盒 Chicken Vascular Endothelial cell Growth Factor,VEGF ELISA Kit Y0302396T/48T
雞免疫球蛋白G(IgG)ELISA試劑盒 Chicken Immunoglobulin G,IgG ELISA Kit Y0302496T/48T
雞堿性成纖維細(xì)胞生長(zhǎng)因子(bFGF)ELISA試劑盒 Chicken basic fibroblast growth factor,bFGF ELISA Kit Y0302596T/48T
雞可溶性細(xì)胞間粘附分子1(sICAM-1)ELISA試劑盒Chicken Soluble Intercellular Adhesion Molecule-1,sICAM-1 ELISA Kit Y0302696T/48T
雞可溶性E選擇素(sE-selectin)ELISA試劑盒 Chicken soluble E-selectin,sE-selectin ELISA Kit Y0302796T/48T
雞表皮生長(zhǎng)因子(EGF)ELISA試劑盒 Chicken Epidermal growth factor,EGF ELISA Kit Y0302896T/48T
雞胰島素樣生長(zhǎng)因子1(IGF-1)ELISA試劑盒 Chicken Insulin-like growth factors 1,IGF-1 ELISA Kit Y0302996T/48T
雞生長(zhǎng)激素(GH)ELISA試劑盒 Chicken growth hormone,GH ELISA Kit Y0303096T/48T
雞乙酰乙酸檢測(cè)(ACAC)ELISA試劑盒Chicken Acetoacetic acid,ACAC ELISA Kit Y0303196T/48T
雞傳染性鼻炎抗體(IC)ELISA試劑盒Chicken infectious coryza,ic ELISA KitY0303296T/48T
雞透明質(zhì)酸(HA)ELISA試劑盒Chicken Hyaluronic acid,HA ELISA KitY0303396T/48T
雞催乳素(PRL)ELISA試劑盒Chicken prolactin,PRL ELISA KitY0303496T/48T
雞白血病病毒抗原(ALV-AG)ELISA試劑盒Chicken Avian Leukosis Virus AG,ALV-AG ELISA KitY0303596T/48T
雞促卵泡素(FSH)ELISA試劑盒Chicken follicle-stimulating hormone,FSH ELISA Kit Y0303696T/48T
雞促黃體激素(LH)ELISA試劑盒Chicken Luteinizing Hormone,LH ELISA KitY0303796T/48T
雞γ干擾素(IFN-γ)ELISA試劑盒 Chicken Interferon γ ,IFN-γ ELISA Kit Y0303896T/48T
雞白介素6(IL-6)ELISA試劑盒Chicken Interleukin 6,IL-6 ELISA KitY0303996T/48T
雞碳酸酐酶(CA)ELISA試劑盒Chicken carbonic anhydrase,CA ELISA KitY0304096T/48T
雞抗凝血酶Ⅲ抗體(AT-Ⅲ)ELISA試劑盒Chicken Anti-Thrombin Ⅲ,AT-Ⅲ ELISA Kit Y0304196T/48T
雞熱休克蛋白20(HSP-20)ELISA試劑盒 Chicken Heat Shock Protein 20,HSP-20 ELISA Kit Y0304296T/48T
雞熱休克蛋白60(Hsp-60)ELISA試劑盒Chicken Heat Shock Protein 60,HSP-60 ELISA Kit Y0304396T/48T
雞17羥皮質(zhì)類固醇(17-OHCS)ELISA試劑盒Hydroxycorticosteroids,17-OHCS ELISA Kit Y0304496T/48T
雞白介素4(IL-4)ELISA試劑盒 Chicken Interleukin 4,IL-4 ELISA Kit Y0304596T/48T
雞白介素2(IL-2)ELISA試劑盒Chicken Interleukin 2,IL-2 ELISA KitY0304696T/48T
雞一氧化氮(NO)ELISA試劑盒Chicken nitric oxide,NO ELISA Kit Y0304796T/48T
雞單純皰疹病毒Ⅱ型抗體(HSVⅡ-Ab)ELISA試劑盒 Chicken Herpes simplexvirus Ⅱ antibody,HSVⅡ-Ab ELISA Kit Y0304896T/48T
雞補(bǔ)體3裂解產(chǎn)物(C3SP)ELISA試劑盒Chicken Complement 3 split product,C3SP ELISA Kit Y0304996T/48T
雞腎上腺髓質(zhì)素(ADM)ELISA試劑盒 Chicken adrenomedullin,ADM ELISA KitY0305096T/48T
各種類型ELISA測(cè)定時(shí), 一般需經(jīng)過(guò)以下步驟:固相包被→加待測(cè)樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。