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小鼠二胺氧化酶酶免試劑盒,(DAO)ELISA檢測試劑盒 ,英文名:DAO ELISA Kit ,英文縮寫:DAO ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠二胺氧化酶ELISA檢測試劑盒,DAO 檢測試劑盒, DAO 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠二胺氧化酶酶免試劑盒,(DAO)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
跨膜蛋白27(TMEM27)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transmembrane Protein 27 (TMEM27)48T/96TY16075Homo sapiens (Human)
肝細(xì)胞生長因子(HGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hepatocyte Growth Factor (HGF)48T/96TY16076Bos taurus; Bovine (Cattle)
煙堿型膽堿受體α1(CHRNα1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1)48T/96TY16077Homo sapiens (Human)
Toll樣受體3(TLR3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 3 (TLR3)48T/96TY16078Mus musculus (Mouse)
絲氨酸/蘇氨酸激酶39(STK39)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Serine/Threonine Kinase 39 (STK39)48T/96TY16079Homo sapiens (Human)
天冬酰胺合成酶(ASNS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Asparagine Synthetase (ASNS)48T/96TY16080Mus musculus (Mouse)
鈣粘蛋白EGF LAG七經(jīng)G-型受體2(CELSR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2)48T/96TY16081Rattus norvegicus (Rat)
彈性蛋白微原纖維界面因子2(EMILIN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Elastin Microfibril Interface Located Protein 2 (EMILIN2)48T/96TY16082Homo sapiens (Human)
絡(luò)絲蛋白(RL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Reelin (RL)48T/96TY16083Rattus norvegicus (Rat)
成纖維細(xì)胞生長因子18(FGF18)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 18 (FGF18)48T/96TY16084Mus musculus (Mouse)
成纖維細(xì)胞生長因子18(FGF18)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 18 (FGF18)48T/96TY16085Rattus norvegicus (Rat)
成纖維細(xì)胞生長因子11(FGF11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 11 (FGF11)48T/96TY16086Rattus norvegicus (Rat)
成纖維細(xì)胞生長因子12(FGF12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 12 (FGF12)48T/96TY16087Rattus norvegicus (Rat)
成纖維細(xì)胞生長因子13(FGF13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 13 (FGF13)48T/96TY16088Rattus norvegicus (Rat)
白介素34(IL34)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 34 (IL34)48T/96TY16089Rattus norvegicus (Rat)
白介素17F(IL17F)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 17F (IL17F)48T/96TY16090Mus musculus (Mouse)
色素上皮衍生因子(PEDF)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Pigment Epithelium Derived Factor (PEDF)48T/96TY16091Rattus norvegicus (Rat)
成纖維細(xì)胞生長因子3(FGF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 3 (FGF3)48T/96TY16092Mus musculus (Mouse)
成纖維細(xì)胞生長因子3(FGF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 3 (FGF3)48T/96TY16093Rattus norvegicus (Rat)
顆粒體蛋白(GRN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Granulin (GRN)48T/96TY16094Mus musculus (Mouse)
顆粒體蛋白(GRN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Granulin (GRN)48T/96TY16095Rattus norvegicus (Rat)
心營養(yǎng)素樣細(xì)胞因子1(CLCF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cardiotrophin Like Cytokine Factor 1 (CLCF1)48T/96TY16096Mus musculus (Mouse)
心營養(yǎng)素樣細(xì)胞因子1(CLCF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cardiotrophin Like Cytokine Factor 1 (CLCF1)48T/96TY16097Rattus norvegicus (Rat)
同種異體移植炎癥因子1(AIF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Allograft Inflammatory Factor 1 (AIF1)48T/96TY16098Rattus norvegicus (Rat)
左右決定因子1(LEFTY1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Left/Right Determination Factor 1 (LEFTY1)48T/96TY16099Mus musculus (Mouse)
左右決定因子1(LEFTY1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Left/Right Determination Factor 1 (LEFTY1)48T/96TY16100Rattus norvegicus (Rat)
Cerberus蛋白(CER)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cerberus (CER)48T/96TY16101Mus musculus (Mouse)
生長分化因子11(GDF11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 11 (GDF11)48T/96TY16102Homo sapiens (Human)
生長分化因子11(GDF11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 11 (GDF11)48T/96TY16103Rattus norvegicus (Rat)
真核翻譯延伸因子1γ(EEF1γ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Eukaryotic Translation Elongation Factor 1 Gamma (EEF1g)48T/96TY16104Homo sapiens (Human)
生長分化因子5(GDF5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 5 (GDF5)48T/96TY16105Mus musculus (Mouse)
生長分化因子5(GDF5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 5 (GDF5)48T/96TY16106Rattus norvegicus (Rat)
生長分化因子3(GDF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 3 (GDF3)48T/96TY16107Homo sapiens (Human)
生長分化因子1(GDF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 1 (GDF1)48T/96TY16108Mus musculus (Mouse)
生長分化因子1(GDF1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Growth Differentiation Factor 1 (GDF1)48T/96TY16109Rattus norvegicus (Rat)
干擾素誘導(dǎo)T-細(xì)胞α亞族趨化劑(ITαC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Inducible T-Cell Alpha Chemoattractant (ITaC)48T/96TY16110Homo sapiens (Human)
干擾素誘導(dǎo)T-細(xì)胞α亞族趨化劑(ITαC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Inducible T-Cell Alpha Chemoattractant (ITaC)48T/96TY16111Mus musculus (Mouse)
VEGF共調(diào)節(jié)趨化因子1(VCC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for VEGF Co Regulated Chemokine 1 (VCC1)48T/96TY16112Mus musculus (Mouse)
VEGF共調(diào)節(jié)趨化因子1(VCC1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for VEGF Co Regulated Chemokine 1 (VCC1)48T/96TY16113Rattus norvegicus (Rat)
白細(xì)胞介素24(IL24)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 24 (IL24)48T/96TY16114Rattus norvegicus (Rat)
神經(jīng)元正五聚蛋白Ⅱ(NPTX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Neuronal Pentraxin II (NPTX2)48T/96TY16115Rattus norvegicus (Rat)
羧肽酶N1(CPN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carboxypeptidase N1 (CPN1)48T/96TY16116Mus musculus (Mouse)
緩激肽受體B2(BDKRB2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Bradykinin Receptor B2 (BDKRB2)48T/96TY16117Rattus norvegicus (Rat)
含BED型鋅指蛋白3(ZBED3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Zinc Finger, BED Type Containing Protein 3 (ZBED3)48T/96TY16118Homo sapiens (Human)
白細(xì)胞彈性蛋白酶抑制因子(LEI)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukocyte Elastase Inhibitor (LEI)48T/96TY16119Homo sapiens (Human)
骨橋素(OPN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Osteopontin (OPN)48T/96TY16120Bos taurus; Bovine (Cattle)
天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Aspartate Aminotransferase (AST)48T/96TY16121Bos taurus; Bovine (Cattle)
蛋白激酶Bβ(PKBβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase B Beta (PKBb)48T/96TY16122Rattus norvegicus (Rat)
帕金森氏病蛋白2(PARK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Parkinson Disease Protein 2 (PARK2)48T/96TY16123Homo sapiens (Human)
鈣蛋白酶2(CAPN2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calpain 2, Large Subunit (CAPN2)48T/96TY16124Homo sapiens (Human)
內(nèi)切核酸酶G(ENDOG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endonuclease G, Mitochondrial (ENDOG)48T/96TY16125Homo sapiens (Human)
甲基CpG結(jié)合蛋白2(MECP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Methyl CpG Binding Protein 2 (MECP2)48T/96TY16126Mus musculus (Mouse)
Apelin 17蛋白(AP17)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apelin 17 (AP17)48T/96TY16127Homo sapiens (Human)
小亞基鈣蛋白酶1(CAPNS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calpain, Small Subunit 1 (CAPNS1)48T/96TY16128Homo sapiens (Human)
絲氨酸棕櫚酰轉(zhuǎn)移酶長鏈堿性亞基3(SPTLC3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Serine Palmitoyltransferase, Long Chain Base Subunit 3 (SPTLC3)48T/96TY16129Homo sapiens (Human)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。