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小鼠L選擇素酶免試劑盒,(L-Selectin/CD62L)ELISA檢測試劑盒,英文名:L-Selectin/CD62L ELISA Kit,英文縮寫:L-Selectin/CD62L ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠L選擇素ELISA檢測試劑盒,L-Selectin/CD62L 檢測試劑盒,L-Selectin/CD62L 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠L選擇素酶免試劑盒,(L-Selectin/CD62L)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
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谷丙轉(zhuǎn)氨酶(ALT)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Alanine Aminotransferase (ALT)96TY17700Rattus norvegicus (Rat)
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紅細胞分化去核相關因子1(EDDF1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Erythroid Differentiation And Denucleation Factor 1 (EDDF1)96TY17705Rattus norvegicus (Rat)
紅細胞分化相關因子1(EDRF1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Erythroid Differentiation Related Factor 1 (EDRF1)96TY17706Homo sapiens (Human)
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轉(zhuǎn)錄延伸因子A樣蛋白3(TCEAL3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Transcription Elongation Factor A Like Protein 3 (TCEAL3)96TY17715Mus musculus (Mouse)
轉(zhuǎn)錄延伸因子A樣蛋白3(TCEAL3)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Transcription Elongation Factor A Like Protein 3 (TCEAL3)96TY17716Rattus norvegicus (Rat)
轉(zhuǎn)錄延伸因子A樣蛋白2(TCEAL2)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Transcription Elongation Factor A Like Protein 2 (TCEAL2)96TY17717Homo sapiens (Human)
神經(jīng)元衍生神經(jīng)營養(yǎng)因子(NDNF)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Neuron Derived Neurotrophic Factor (NDNF)96TY17718Mus musculus (Mouse)
纖維膠凝蛋白1(FCN1)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Ficolin 1 (FCN1)96TY17719Rattus norvegicus (Rat)
銅藍蛋白(CP)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Ceruloplasmin (CP)96TY17720Rattus norvegicus (Rat)
鈣網(wǎng)蛋白(CRT)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Calreticulin (CRT)96TY17721Rattus norvegicus (Rat)
載脂蛋白B48(APOB48)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Apolipoprotein B48 (APOB48)96TY17722Homo sapiens (Human)
硬骨素(SOST)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Sclerostin (SOST)96TY17723Rattus norvegicus (Rat)
成纖維細胞生長因子18(FGF18)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 18 (FGF18)96TY17724Mus musculus (Mouse)
成纖維細胞生長因子18(FGF18)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 18 (FGF18)96TY17725Rattus norvegicus (Rat)
成纖維細胞生長因子18(FGF18)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 18 (FGF18)96TY17726Homo sapiens (Human)
成纖維細胞生長因子11(FGF11)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 11 (FGF11)96TY17727Homo sapiens (Human)
成纖維細胞生長因子11(FGF11)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 11 (FGF11)96TY17728Mus musculus (Mouse)
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成纖維細胞生長因子12(FGF12)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 12 (FGF12)96TY17731Mus musculus (Mouse)
成纖維細胞生長因子12(FGF12)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 12 (FGF12)96TY17732Rattus norvegicus (Rat)
成纖維細胞生長因子13(FGF13)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 13 (FGF13)96TY17733Homo sapiens (Human)
成纖維細胞生長因子13(FGF13)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 13 (FGF13)96TY17734Mus musculus (Mouse)
成纖維細胞生長因子13(FGF13)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Fibroblast Growth Factor 13 (FGF13)96TY17735Rattus norvegicus (Rat)
白介素34(IL34)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 34 (IL34)96TY17736Rattus norvegicus (Rat)
白介素28B(IL28B)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 28B (IL28B)96TY17737Homo sapiens (Human)
白介素17F(IL17F)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 17F (IL17F)96TY17738Homo sapiens (Human)
白介素17F(IL17F)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 17F (IL17F)96TY17739Mus musculus (Mouse)
白介素17F(IL17F)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 17F (IL17F)96TY17740Rattus norvegicus (Rat)
色素上皮衍生因子(PEDF)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Pigment Epithelium Derived Factor (PEDF)96TY17741Homo sapiens (Human)
色素上皮衍生因子(PEDF)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Pigment Epithelium Derived Factor (PEDF)96TY17742Mus musculus (Mouse)
色素上皮衍生因子(PEDF)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Pigment Epithelium Derived Factor (PEDF)96TY17743Rattus norvegicus (Rat)
白介素33(IL33)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 33 (IL33)96TY17744Homo sapiens (Human)
白介素33(IL33)檢測試劑盒(化學發(fā)光免疫分析法)CLIA Kit for Interleukin 33 (IL33)96TY17745Mus musculus (Mouse)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。