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細(xì)胞中文名稱SV-40Z轉(zhuǎn)化肺成纖維細(xì)胞
細(xì)胞英文名稱WI38/VA13
物種
細(xì)胞形態(tài)特征上皮細(xì)胞樣
細(xì)胞生長特性SV-40Z轉(zhuǎn)化肺成纖維細(xì)胞WI38VA13細(xì)胞 貼壁生長
細(xì)胞特種特性該細(xì)胞1964年建系,源自3個(gè)月胎齡的女性胚胎的肺成纖維細(xì)胞,經(jīng)SV-40轉(zhuǎn)化
培養(yǎng)基MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts
血清10%FBS
細(xì)胞傳代方法1:2~1:10傳代,每周換液2次
細(xì)胞傳代情況C2
細(xì)胞凍存條件基礎(chǔ)培養(yǎng)基+8%DMSO+20%FBS
支原體檢測培養(yǎng)法(-)
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